Download >>> https://byltly.com/282fm5
In this post, we will be talking about the matter of how to go about extracting the all-important DNA from a simple fruit fly. This can come in handy when you want to have an impact on a famous species or recreate a species that has been extinct for many years. Now let's get started! The first step is to get your tools ready. The most important thing you need is being able to see what you're doing, so invest in some good light if possible. You'll also need a sharp razor blade or scalpel and some rubbing alcohol because DNA doesn't dissolve well in water and could cause your jar lid to get stuck shut, which would be frustrating. In the following steps, we will be using a "wet mount" form of observation, so if you have a cover slip or slide handy so that you can place your specimen on it. Don't forget to have an extra slide just in case! Now that you're ready, let's get started. First things first, you need a sample of some fruit flies. You can gather these from around your house or purchase them online from almost any scientific supply store. Generally speaking, the species of species that the flies belong to is inconsequential as long as you make sure they are healthy and good specimens. In this example, we'll be using yellow- Labyrinth Fruit Flies (Drosophila melanogaster). They are very small, but quite prominent-looking. Alright, now that you have your flies, you'll want to kill the specimens. Fruit flies are not very happy about being trapped inside a jar, so they will try to escape. Killing them humanely is important because you don't want any suffering to occur, since DNA extraction isn't exactly a quick process. The best way to kill them is with rubbing alcohol. Simply shake the flies around in the jar until they go still and then dump out the contents of the jar onto a slide or other platform for observation. Then, spill some rubbing alcohol over the top and make sure it is thoroughly soaked up. Next, you'll want to get your extractor or extractor-generator and place it in a 3.5-5.0 mL plastic tube with cap on it to prevent any spills and breakage. Ensure that the inner sphere of the canister is full of crushed ice or frozen water so that when you press down on the roller plate, the liquid inside will stay cold without freezing solid. After the extractor is set up, fill it about 1/2 full of ice. Now, you'll want to get your glassware ready by placing the bottom into a pan of ice water for an hour or two. You can also cover it with cling wrap, but this isn't necessary. Next, you'll want to get your lids ready because you will need them both to keep the specimens in place while extracting their DNA. You'll want something that has a good seal so that the lid doesn't leak when you try to remove or replace it later on. cfa1e77820
Comentários